Primer
Part:BBa_K2973017:Design
Designed by: Nick Delkis, Aphrodite Katsaouni Group: iGEM19_Thessaly (2019-09-28)
IS6110 Reverse Primer with 5' Overhang
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The primer design was accomplished using the Primer3 software (Primer-BLAST). The trigger sequence on the 5' end of the sequence allows for downstream translation by both in vitro (cell-free) and in vivo systems.
Source
NCBI Mycobacterium tuberculosis Genome, Strain H37Rv, Accession Number: NC_000962